Spiral microfluidic devices for cell separation and sorting in bioprocesses

Continuous separation of blood cells in spiral microfluidic devices.

Blood cell sorting is critical to sample preparation for both clinical diagnosis and therapeutic research. The spiral inertial microfluidic devices can achieve label-free, continuous separation of cell mixtures with high throughput and efficiency. The devices utilize hydrodynamic forces acting on cells within laminar flow, coupled with rotational Dean drag due to curvilinear microchannel geomet...

Fluorescence-Activated Cell Sorting in Microfluidic Devices

Label-free cell separation and sorting in microfluidic systems

Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods. Recently, there has been interest in methods which avoid the use of biochemical labels; numerous intrinsic biomarkers have been explored to identify cells including size, electrical polarizability, and hydrodynamic properties. This review highlights microfluidic techniques us...

Automated Dielectrophoretic Characterization for Microfluidic Cell Separation Devices

We present a novel analysis technique for automated characterization of particles and cells using dielectrophoresis (DEP). We combine an automated experimental system to measure DEP particle trapping via fluorescence intensity with physically informed computational methods and sophisticated particle models to make robust measurements of DEP force magnitude. The system uses a LabVIEW-controlled ...

Manufacturing and wetting low-cost microfluidic cell separation devices.

Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a simple, yet robust protocol for thermoplastic DLD devices using regulatory-appr...